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Peptide fibrils as a vaccine: Proof of concept
The availability of platforms that enable the rapid development of a vaccine to protect against pathogens causing local ou...
Publisher's note: Challenges for complement functional assays in the clinical laboratory: From test validation to clinical interpretation
Volume 538, March 2025, 113814Author links open overlay panelVijayalakshmi Nandakumar a, Karin M.P. Braun b, Maria Alice V...
Overcoming cross-reactivity of antibodies against human lactate dehydrogenase
Lactate dehydrogenase (LD) is a key enzyme involved in glycolysis under anaerobic conditions, converting a glucose molecul...
Complete primer set for amplification and expression of full-length recombinant human monoclonal antibodies from single human B cells
B cells play an important role in combating infections. The humoral immune response is initiated when a B cell encounters ...
Complement function and activation in human serum and plasma collected in different blood collection tubes
Volume 538, March 2025, 113825Author links open overlay panel, , , , , Highlights•The type of collection tube significantl...
Comprehensive normalization and binary classification methods for enhanced sensitivity and reproducibility in Luminex assay quantitation
The Luminex assay is a versatile and widely used platform for large-scale analyte quantification, such as the quantificati...
Preparation and specific identification of monoclonal antibodies targeting the VP2 structural protein of minute virus of canines
Minute Virus of Canines (MVC) was first isolated from canine fecal samples in 1967 (Binn et al., 1967). Since its discover...
MARMOT: A multifaceted R pipeline for analysing spectral flow cytometry data from subcutaneously growing murine gastric organoids
The analysis of murine immune cell types is a critical component of immunological research, necessitating precise and repr...
Corrigendum to “Comprehensive normalization and binary classification methods for enhanced sensitivity and reproducibility in Luminex assay quantitation” [Journal of Immunological Methods 538 (2025) 113826]
Available online 22 March 2025, 113852Author links open overlay panelB.A. Burns a, C.A. Shaw b c, M. Chandra a, C.S. Forco...
Identification of polyreactive antibodies by high throughput enzyme-linked immunosorbent assay and surface Plasmon resonance
As therapeutics, antibodies stand out for their specificities, affinities, biological activities, expression levels, stabi...
Optimization of blood sampling time for isolation of antigen binding single-domain antibodies from immunized llamas
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Highly sensitive detection of carcinoembryonic antigen via an electrochemical platform fabricated by CCLP- gold nanocomposite
Cancer biomarker is a significant indicator to establish the presence of a disease or to monitor the therapeutic effect of...
Chimeric protein A/G conjugate based lateral flow assay for the rapid detection of brucellosis in multiple animal species
Brucellosis is an epizootic disease that needs great attention due to its high zoonotic potential (Natesan et al., 2021). ...
Anti-chicken and anti-pigeon immunoglobulin G testing in patients with bird-related fibrotic hypersensitivity pneumonitis
Inciting antigens for bird-related hypersensitivity pneumonitis (HP) or bird fancier's lung have been reported in avian se...
CHO-S expression of a novel human recombinant IgG1 of anti-ALD antibody isolated by phage mutation display
Aldosterone (ALD), a hormone that regulates sodium balance and blood pressure in humans and a steroid hormone, also has a ...
ELISA protein detector (EPD): A Python-based ELISA tool for accurate low-level protein quantification
The enzyme-linked immunosorbent assay (ELISA) has been a transformative tool (Clark et al., 1986) in biomedical and clinic...
NADPH oxidase subunit p22phox: A marker of oxidase-dependent oxidative stress and target for stress suppression in nonphagocytic cells
The reactive oxygen species (ROS)-producing NADPH oxidase (Nox) family plays important roles in host defense (Song et al.,...
The generation of a novel HER2-targeting nano PE25 immunotoxin with superior anti-tumor activity and high productivity
Human epidermal growth factor receptor 2 (HER2) is one of epidermal growth factor receptor family members that is overexpr...
Optimization of IgG1 immune complexes to stimulate cytokine production in innate cells
The immunoglobulin family plays a crucial role in the body's immune defense mechanisms. IgG is the most abundant subclass ...
Flow cytometry reveals constant lymphocyte proportions after long-term cryopreservation of whole blood in TransFix® cell stabilization reagent
Volume 539, April 2025, 113853Author links open overlay panel, , , Highlights•Extends prior findings on short-term to long...
Biodegradable Zein/PEG Nanofibers Incorporated with Natural Antimicrobial Compounds for Eco-Friendly Food Packaging
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Enhancing 2,3-butanediol and acetoin production from brewer's spent grain hemicellulosic hydrolysate through bacterial co-cultivation
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Biosynthesis of biodegradable plastic, poly(3HB-co-3HP), using methane as sole carbon source in metabolically engineered type II methanotroph, Methylosinus trichosporium OB3b
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Microfluidic method for rapidly determining the protein and lipid yield of microalgae
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Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol production
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Enzyme-assisted extraction and product profiling of fucosylated xyloglucan from industrial citrus pectin residues
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OncoFlow: A multiplexed microfluidic platform for personalized drug sensitivity assessment
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From the lab to the field and closer to the market: Production of the biopolymer cyanophycin in plants
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Microalgal biorefineries in sustainable biofuel production and other high-value products
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Antibacterial and biodegradable PLA-based nanofibers loaded with natural phenolic monoterpenes for sustainable biomedical or food application
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